Liquid Chromatographic Analysis of Vitamin K1 in Milk-Based Infant Formula with Matrix Solid-Phase Dispersion

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Liquid chromatographic analysis of vitamin K1 in milk-based infant formula with matrix solid-phase dispersion.

A liquid chromatographic method for vitamin K1 in milk-based infant formula is described. The vitamins are extracted from infant formula by matrix solid-phase dispersion and quantitated by reversed-phase chromatography with fluorescence detection. Vitamin K1 is converted to the fluorescent hydroquinone with a postcolumn zinc reductive reactor. The limit of detection is 12 pg, and the limit of q...

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Accelerated solvent extraction of vitamin K1 in medical foods in conjunction with matrix solid-phase dispersion.

An extraction technique is described for vitamin K1 in medical foods, using accelerated solvent extraction (ASE) in conjunction with matrix solid-phase dispersion (MSPD). The medical food sample is treated as it would be with MSPD extraction, followed by ASE for a hands-free automated extraction. The vitamin K1 in the ASE extract is then quantitated by reversed-phase liquid chromatography with ...

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Liquid chromatographic analysis of vitamin B6 in soy-based infant formula.

A liquid chromatographic (LC) method is described for determination of total vitamin B6 in soy-based infant formula. Total vitamin B6 is quantitated by using ion-pair LC after precolumn transformation of phosphorylated and free vitamers into pyridoxol. The limit of detection is 0.3 ng and the limit of quantitation is 1.0 ng on-column (injection volume = 100 microL). Linear response ranged from ...

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Matrix solid phase dispersion (MSPD).

A review of the many uses of matrix solid phase dispersion (MSPD) in the extraction and analysis of a variety of compounds from a range of samples is provided. Matrix solid phase dispersion (MSPD) has found particular application as a somewhat generic analytical process for the preparation, extraction and fractionation of solid, semi-solid and/or highly viscous biological samples. Its simplicit...

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Simultaneous liquid-chromatographic determination of vitamin K1 and vitamin E in serum.

We describe a high-performance liquid chromatographic procedure for the simultaneous measurement of vitamins K1 and E in human serum. Delipidated human serum (free of vitamins K1 and E) was used to make standard solutions of these vitamins, and cetyl naphthoate and alpha-tocopheryl acetate were the internal standards for vitamin K1 and vitamin E, respectively. A simple, novel separation method ...

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ژورنال

عنوان ژورنال: Journal of AOAC INTERNATIONAL

سال: 1999

ISSN: 1060-3271,1944-7922

DOI: 10.1093/jaoac/82.5.1140